Expression, purification, crystallization and preliminary crystallographic analysis of BipD, a component of the Burkholderia pseudomallei type III secretion system

A construct consisting of residues 10–310 of BipD, a component of the Burkholderia pseudomallei type III secretion system (T3SS), has been overexpressed as a GST fusion, cleaved from the GST tag and purified. Crystals were grown of native and selenomethionine-labelled BipD. The crystals grow in two...

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Detalles Bibliográficos
Autores principales: Roversi, Pietro, Johnson, Steven, Field, Terry, Deane, Janet E., Galyov, Edouard E., Lea, Susan M.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2006
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894745/
https://www.ncbi.nlm.nih.gov/pubmed/16946464
http://dx.doi.org/10.1107/S1744309106027035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894745/
https://www.ncbi.nlm.nih.gov/pubmed/16946464
http://dx.doi.org/10.1107/S1744309106027035

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