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The role of CDK in the initiation step of DNA replication in eukaryotes

Cyclin-dependent kinases (CDKs) regulate the progression of the cell cycle in eukaryotes. One of the major roles of CDK is to promote chromosomal DNA replication. However, how CDKs promote DNA replication has been a long-standing question, because all the essential CDK substrates in DNA replication...

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Detalles Bibliográficos
Autores principales: Tanaka, Seiji, Tak, Yon-Soo, Araki, Hiroyuki
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1899495/
https://www.ncbi.nlm.nih.gov/pubmed/17547773
http://dx.doi.org/10.1186/1747-1028-2-16
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author Tanaka, Seiji
Tak, Yon-Soo
Araki, Hiroyuki
author_facet Tanaka, Seiji
Tak, Yon-Soo
Araki, Hiroyuki
author_sort Tanaka, Seiji
collection PubMed
description Cyclin-dependent kinases (CDKs) regulate the progression of the cell cycle in eukaryotes. One of the major roles of CDK is to promote chromosomal DNA replication. However, how CDKs promote DNA replication has been a long-standing question, because all the essential CDK substrates in DNA replication have not been identified yet. Recently Sld2 and Sld3 were identified as essential substrates of CDKs in the initiation step of DNA replication in budding yeast. Moreover, bypass of their phosphorylations is sufficient to promote DNA replication. Phosphorylation of Sld2 and Sld3 by CDKs enhances the formation of complex(es) with a BRCT (BRCA1 C-Terminal)-containing replication protein, Dpb11. We further propose that multiple phosphorylation by CDKs controls this process in budding yeast. Even though Sld3 orthologues in multicellular eukaryotes have not been identified, similar complex formation and, therefore, a similar mechanism of initiation control might be employed in eukaryotes.
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spelling pubmed-18994952007-06-27 The role of CDK in the initiation step of DNA replication in eukaryotes Tanaka, Seiji Tak, Yon-Soo Araki, Hiroyuki Cell Div Commentary Cyclin-dependent kinases (CDKs) regulate the progression of the cell cycle in eukaryotes. One of the major roles of CDK is to promote chromosomal DNA replication. However, how CDKs promote DNA replication has been a long-standing question, because all the essential CDK substrates in DNA replication have not been identified yet. Recently Sld2 and Sld3 were identified as essential substrates of CDKs in the initiation step of DNA replication in budding yeast. Moreover, bypass of their phosphorylations is sufficient to promote DNA replication. Phosphorylation of Sld2 and Sld3 by CDKs enhances the formation of complex(es) with a BRCT (BRCA1 C-Terminal)-containing replication protein, Dpb11. We further propose that multiple phosphorylation by CDKs controls this process in budding yeast. Even though Sld3 orthologues in multicellular eukaryotes have not been identified, similar complex formation and, therefore, a similar mechanism of initiation control might be employed in eukaryotes. BioMed Central 2007-06-05 /pmc/articles/PMC1899495/ /pubmed/17547773 http://dx.doi.org/10.1186/1747-1028-2-16 Text en Copyright © 2007 Tanaka et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Commentary
Tanaka, Seiji
Tak, Yon-Soo
Araki, Hiroyuki
The role of CDK in the initiation step of DNA replication in eukaryotes
title The role of CDK in the initiation step of DNA replication in eukaryotes
title_full The role of CDK in the initiation step of DNA replication in eukaryotes
title_fullStr The role of CDK in the initiation step of DNA replication in eukaryotes
title_full_unstemmed The role of CDK in the initiation step of DNA replication in eukaryotes
title_short The role of CDK in the initiation step of DNA replication in eukaryotes
title_sort role of cdk in the initiation step of dna replication in eukaryotes
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1899495/
https://www.ncbi.nlm.nih.gov/pubmed/17547773
http://dx.doi.org/10.1186/1747-1028-2-16
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