An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities

Determination of disaccharidase and glucoamylase activities is important for the diagnosis of intestinal diseases. We adapted a widely accepted manual method to an automated system that uses the same reagents reaction volumes, incubation times, and biopsy size as the manual method. A dye was added t...

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Autores principales: He, Zhaoping, Bolling, Laura, Tonb, Dalal, Nadal, Tracey, Mehta, Devendra I.
Formato: Texto
Lenguaje:English
Publicado: 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1903450/
https://www.ncbi.nlm.nih.gov/pubmed/17671629
http://dx.doi.org/10.1155/JAMMC/2006/93947
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author He, Zhaoping
Bolling, Laura
Tonb, Dalal
Nadal, Tracey
Mehta, Devendra I.
author_facet He, Zhaoping
Bolling, Laura
Tonb, Dalal
Nadal, Tracey
Mehta, Devendra I.
author_sort He, Zhaoping
collection PubMed
description Determination of disaccharidase and glucoamylase activities is important for the diagnosis of intestinal diseases. We adapted a widely accepted manual method to an automated system that uses the same reagents reaction volumes, incubation times, and biopsy size as the manual method. A dye was added to the homogenates as the internal quality control to monitor the pipetting precision of the automated system. When the automated system was tested using human intestinal homogenates, the activities of all the routinely tested disaccharidases, including lactase, maltase, sucrase, and palatinase, as well as the activity of glucoamylase, showed perfect agreement with the manual method and were highly reproducible. The automated analyzer can perform the same routine assays of disaccharidases and glucoamylase with high consistency and accuracy and reduce testing costs by performing a larger sample size with the same number of staff. Additional developments, such as barcoding and built-in plate reading, would result in a completely automated system.
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spelling pubmed-19034502007-08-01 An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities He, Zhaoping Bolling, Laura Tonb, Dalal Nadal, Tracey Mehta, Devendra I. J Autom Methods Manag Chem Research Article Determination of disaccharidase and glucoamylase activities is important for the diagnosis of intestinal diseases. We adapted a widely accepted manual method to an automated system that uses the same reagents reaction volumes, incubation times, and biopsy size as the manual method. A dye was added to the homogenates as the internal quality control to monitor the pipetting precision of the automated system. When the automated system was tested using human intestinal homogenates, the activities of all the routinely tested disaccharidases, including lactase, maltase, sucrase, and palatinase, as well as the activity of glucoamylase, showed perfect agreement with the manual method and were highly reproducible. The automated analyzer can perform the same routine assays of disaccharidases and glucoamylase with high consistency and accuracy and reduce testing costs by performing a larger sample size with the same number of staff. Additional developments, such as barcoding and built-in plate reading, would result in a completely automated system. 2006 /pmc/articles/PMC1903450/ /pubmed/17671629 http://dx.doi.org/10.1155/JAMMC/2006/93947 Text en Copyright © 2006 Zhaoping He et al. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
He, Zhaoping
Bolling, Laura
Tonb, Dalal
Nadal, Tracey
Mehta, Devendra I.
An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities
title An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities
title_full An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities
title_fullStr An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities
title_full_unstemmed An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities
title_short An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities
title_sort automated method for the determination of intestinal disaccharidase and glucoamylase activities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1903450/
https://www.ncbi.nlm.nih.gov/pubmed/17671629
http://dx.doi.org/10.1155/JAMMC/2006/93947
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