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The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage
Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel perspectives for genome engineering in a wide range of fields, including gene therapy. A new crystal struc...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904291/ https://www.ncbi.nlm.nih.gov/pubmed/17452357 http://dx.doi.org/10.1093/nar/gkm183 |
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author | Prieto, Jesús Redondo, Pilar Padró, Daniel Arnould, Sylvain Epinat, Jean-Charles Pâques, Frédéric Blanco, Francisco J. Montoya, Guillermo |
author_facet | Prieto, Jesús Redondo, Pilar Padró, Daniel Arnould, Sylvain Epinat, Jean-Charles Pâques, Frédéric Blanco, Francisco J. Montoya, Guillermo |
author_sort | Prieto, Jesús |
collection | PubMed |
description | Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel perspectives for genome engineering in a wide range of fields, including gene therapy. A new crystal structure of the I-CreI dimer without DNA has allowed the comparison with the DNA-bound protein. The C-terminal loop displays a different conformation, which suggests its implication in DNA binding. A site-directed mutagenesis study in this region demonstrates that whereas the C-terminal helix is negligible for DNA binding, the final C-terminal loop is essential in DNA binding and cleavage. We have identified two regions that comprise the Ser138–Lys139 and Lys142–Thr143 pairs whose double mutation affect DNA binding in vitro and abolish cleavage in vivo. However, the mutation of only one residue in these sites allows DNA binding in vitro and cleavage in vivo. These findings demonstrate that the C-terminal loop of I-CreI endonuclease plays a fundamental role in its catalytic mechanism and suggest this novel site as a region to take into account for engineering new endonucleases with tailored specificity. |
format | Text |
id | pubmed-1904291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-19042912007-07-03 The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage Prieto, Jesús Redondo, Pilar Padró, Daniel Arnould, Sylvain Epinat, Jean-Charles Pâques, Frédéric Blanco, Francisco J. Montoya, Guillermo Nucleic Acids Res Structural Biology Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel perspectives for genome engineering in a wide range of fields, including gene therapy. A new crystal structure of the I-CreI dimer without DNA has allowed the comparison with the DNA-bound protein. The C-terminal loop displays a different conformation, which suggests its implication in DNA binding. A site-directed mutagenesis study in this region demonstrates that whereas the C-terminal helix is negligible for DNA binding, the final C-terminal loop is essential in DNA binding and cleavage. We have identified two regions that comprise the Ser138–Lys139 and Lys142–Thr143 pairs whose double mutation affect DNA binding in vitro and abolish cleavage in vivo. However, the mutation of only one residue in these sites allows DNA binding in vitro and cleavage in vivo. These findings demonstrate that the C-terminal loop of I-CreI endonuclease plays a fundamental role in its catalytic mechanism and suggest this novel site as a region to take into account for engineering new endonucleases with tailored specificity. Oxford University Press 2007-05 2007-04-22 /pmc/articles/PMC1904291/ /pubmed/17452357 http://dx.doi.org/10.1093/nar/gkm183 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Structural Biology Prieto, Jesús Redondo, Pilar Padró, Daniel Arnould, Sylvain Epinat, Jean-Charles Pâques, Frédéric Blanco, Francisco J. Montoya, Guillermo The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage |
title | The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage |
title_full | The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage |
title_fullStr | The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage |
title_full_unstemmed | The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage |
title_short | The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage |
title_sort | c-terminal loop of the homing endonuclease i-crei is essential for site recognition, dna binding and cleavage |
topic | Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904291/ https://www.ncbi.nlm.nih.gov/pubmed/17452357 http://dx.doi.org/10.1093/nar/gkm183 |
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