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A facile method for attaching nitroxide spin labels at the 5′ terminus of nucleic acids(†)
In site-directed spin labeling (SDSL), a nitroxide moiety containing a stable, unpaired electron is covalently attached to a specific site within a macromolecule, and structural and dynamic information at the labeling site is obtained via electron paramagnetic resonance (EPR) spectroscopy. Successfu...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904292/ https://www.ncbi.nlm.nih.gov/pubmed/17517787 http://dx.doi.org/10.1093/nar/gkm240 |