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Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast

Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protei...

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Detalles Bibliográficos
Autores principales: Gupta, Ronish, Kus, Bart, Fladd, Christopher, Wasmuth, James, Tonikian, Raffi, Sidhu, Sachdev, Krogan, Nevan J, Parkinson, John, Rotin, Daniela
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1911201/
https://www.ncbi.nlm.nih.gov/pubmed/17551511
http://dx.doi.org/10.1038/msb4100159