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Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast
Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protei...
| Autores principales: | , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2007
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1911201/ https://www.ncbi.nlm.nih.gov/pubmed/17551511 http://dx.doi.org/10.1038/msb4100159 |