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Regulation of DNA nucleases by molecular crowding

Here, we examined the effects of molecular crowding on the function, structure and stability of nucleases. We found that the hydrolysis of a 29-mer double-stranded DNA by the endonucleases DNase I and S1 nuclease was substantially enhanced by molecular crowding using polyethylene glycol (PEG); howev...

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Detalles Bibliográficos
Autores principales: Sasaki, Yoshiharu, Miyoshi, Daisuke, Sugimoto, Naoki
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1919490/
https://www.ncbi.nlm.nih.gov/pubmed/17567601
http://dx.doi.org/10.1093/nar/gkm445