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Using Single loxP Sites to Enhance Homologous Recombination: ts Mutants in Sec1 of Dictyostelium discoideum

BACKGROUND: Dictyostelium discoideum amoebae are haploid and, as they share many features with animal cells, should be an ideal creature for studying basic processes such as cell locomotion. Isolation of mutants in this amoeba has largely been limited to non-essential genes: nsfA—the gene for NEM-se...

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Detalles Bibliográficos
Autores principales: Bretscher, Mark S., Clotworthy, Margaret
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1933600/
https://www.ncbi.nlm.nih.gov/pubmed/17684569
http://dx.doi.org/10.1371/journal.pone.0000724