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Analytical biochemistry of DNA–protein assemblies from crude cell extracts

Purification of specific DNA–protein complexes is a challenging task, as the involved interactions can be both electrostatic/H-bond and hydrophobic. The chromatographic stringency needed to obtain reasonable purifications uses salts and detergents. However, these components elicit the removal of pro...

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Detalles Bibliográficos
Autores principales: Hégarat, Nadia, Cardoso, Gildas Mouta, Rusconi, Filippo, François, Jean-Christophe, Praseuth, Danièle
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1935021/
https://www.ncbi.nlm.nih.gov/pubmed/17617645
http://dx.doi.org/10.1093/nar/gkm490