Cargando…
Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors
We investigated the impact of curcumin on neutrophils. Chemotactic activity via human recombinant IL-8 (hrIL-8) was significantly inhibited by curcumin. Curcumin reduced calcium ion flow induced by internalization of the IL-8 receptor. We analyzed flow cytometry to evaluate the status of the IL-8 re...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1940327/ https://www.ncbi.nlm.nih.gov/pubmed/17710245 http://dx.doi.org/10.1155/2007/10767 |
_version_ | 1782134462669127680 |
---|---|
author | Takahashi, Masafumi Ishiko, Takatoshi Kamohara, Hidenobu Hidaka, Hideaki Ikeda, Osamu Ogawa, Michio Baba, Hideo |
author_facet | Takahashi, Masafumi Ishiko, Takatoshi Kamohara, Hidenobu Hidaka, Hideaki Ikeda, Osamu Ogawa, Michio Baba, Hideo |
author_sort | Takahashi, Masafumi |
collection | PubMed |
description | We investigated the impact of curcumin on neutrophils. Chemotactic activity via human recombinant IL-8 (hrIL-8) was significantly inhibited by curcumin. Curcumin reduced calcium ion flow induced by internalization of the IL-8 receptor. We analyzed flow cytometry to evaluate the status of the IL-8 receptor after curcumin treatment. The change in the distribution of receptors intracellularly and on the cell surface suggested that curcumin may affect the receptor trafficking pathway intracellulary. Rab11 is a low molecular weight G protein associated with the CXCR recycling pathway. Following curcumin treatment, immunoprecipitation studies showed that the IL-8 receptor was associated with larger amounts of active Rab11 than that in control cells. These data suggest that curcumin induces the stacking of the Rab11 vesicle complex with CXCR1 and CXCR2 in the endocytic pathway. The mechanism for antiinflammatory response by curcumin may involve unique regulation of the Rab11 trafficking molecule in recycling of IL-8 receptors. |
format | Text |
id | pubmed-1940327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-19403272007-08-20 Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors Takahashi, Masafumi Ishiko, Takatoshi Kamohara, Hidenobu Hidaka, Hideaki Ikeda, Osamu Ogawa, Michio Baba, Hideo Mediators Inflamm Research Article We investigated the impact of curcumin on neutrophils. Chemotactic activity via human recombinant IL-8 (hrIL-8) was significantly inhibited by curcumin. Curcumin reduced calcium ion flow induced by internalization of the IL-8 receptor. We analyzed flow cytometry to evaluate the status of the IL-8 receptor after curcumin treatment. The change in the distribution of receptors intracellularly and on the cell surface suggested that curcumin may affect the receptor trafficking pathway intracellulary. Rab11 is a low molecular weight G protein associated with the CXCR recycling pathway. Following curcumin treatment, immunoprecipitation studies showed that the IL-8 receptor was associated with larger amounts of active Rab11 than that in control cells. These data suggest that curcumin induces the stacking of the Rab11 vesicle complex with CXCR1 and CXCR2 in the endocytic pathway. The mechanism for antiinflammatory response by curcumin may involve unique regulation of the Rab11 trafficking molecule in recycling of IL-8 receptors. Hindawi Publishing Corporation 2007 2007-06-28 /pmc/articles/PMC1940327/ /pubmed/17710245 http://dx.doi.org/10.1155/2007/10767 Text en Copyright © 2007 Masafumi Takahashi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Takahashi, Masafumi Ishiko, Takatoshi Kamohara, Hidenobu Hidaka, Hideaki Ikeda, Osamu Ogawa, Michio Baba, Hideo Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors |
title | Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors |
title_full | Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors |
title_fullStr | Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors |
title_full_unstemmed | Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors |
title_short | Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) Blocks the Chemotaxis of Neutrophils by Inhibiting Signal Transduction through IL-8 Receptors |
title_sort | curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione) blocks the chemotaxis of neutrophils by inhibiting signal transduction through il-8 receptors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1940327/ https://www.ncbi.nlm.nih.gov/pubmed/17710245 http://dx.doi.org/10.1155/2007/10767 |
work_keys_str_mv | AT takahashimasafumi curcumin17bis4hydroxy3methoxyphenyl16heptadiene35dioneblocksthechemotaxisofneutrophilsbyinhibitingsignaltransductionthroughil8receptors AT ishikotakatoshi curcumin17bis4hydroxy3methoxyphenyl16heptadiene35dioneblocksthechemotaxisofneutrophilsbyinhibitingsignaltransductionthroughil8receptors AT kamoharahidenobu curcumin17bis4hydroxy3methoxyphenyl16heptadiene35dioneblocksthechemotaxisofneutrophilsbyinhibitingsignaltransductionthroughil8receptors AT hidakahideaki curcumin17bis4hydroxy3methoxyphenyl16heptadiene35dioneblocksthechemotaxisofneutrophilsbyinhibitingsignaltransductionthroughil8receptors AT ikedaosamu curcumin17bis4hydroxy3methoxyphenyl16heptadiene35dioneblocksthechemotaxisofneutrophilsbyinhibitingsignaltransductionthroughil8receptors AT ogawamichio curcumin17bis4hydroxy3methoxyphenyl16heptadiene35dioneblocksthechemotaxisofneutrophilsbyinhibitingsignaltransductionthroughil8receptors AT babahideo curcumin17bis4hydroxy3methoxyphenyl16heptadiene35dioneblocksthechemotaxisofneutrophilsbyinhibitingsignaltransductionthroughil8receptors |