Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins

BACKGROUND: Expression of higher eukaryotic genes as soluble, stable recombinant proteins is still a bottleneck step in biochemical and structural studies of novel proteins today. Correct identification of stable domains/fragments within the open reading frame (ORF), combined with proper cloning str...

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Detalles Bibliográficos
Autores principales: Chen, Yunjia, Qiu, Shihong, Luan, Chi-Hao, Luo, Ming
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950093/
https://www.ncbi.nlm.nih.gov/pubmed/17663785
http://dx.doi.org/10.1186/1472-6750-7-45

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950093/
https://www.ncbi.nlm.nih.gov/pubmed/17663785
http://dx.doi.org/10.1186/1472-6750-7-45

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