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The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro.
The effect of temperature on the cytotoxicity of melphalan in a pleiotropic drug-resistant mutant CHO cell line (CHR C5) and in its drug-sensitive parent (Aux B1) was studied in vitro using a clonogenic assay. The cytotoxicity of melphalan was significantly enhanced at elevated but non-lethal temper...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971810/ https://www.ncbi.nlm.nih.gov/pubmed/1974814 |
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author | Bates, D. A. Mackillop, W. J. |
author_facet | Bates, D. A. Mackillop, W. J. |
author_sort | Bates, D. A. |
collection | PubMed |
description | The effect of temperature on the cytotoxicity of melphalan in a pleiotropic drug-resistant mutant CHO cell line (CHR C5) and in its drug-sensitive parent (Aux B1) was studied in vitro using a clonogenic assay. The cytotoxicity of melphalan was significantly enhanced at elevated but non-lethal temperatures (39-41 degrees C) and hyperthermia potentiated the effect of melphalan in the lethal temperature range (43-44 degrees C) in both cell lines. The effect of temperature on membrane permeability to melphalan was studied to determine whether the increase in cytotoxicity was associated with increased intracellular drug levels. The uptake of 14C-labelled melphalan during 5 min increased with increasing temperature. Drug efflux, however, also increased at elevated temperatures. Intracellular drug levels at equilibrium were increased at elevated temperatures but the magnitude of this effect was small in comparison with the much larger increases in cytotoxicity. |
format | Text |
id | pubmed-1971810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19718102009-09-10 The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro. Bates, D. A. Mackillop, W. J. Br J Cancer Research Article The effect of temperature on the cytotoxicity of melphalan in a pleiotropic drug-resistant mutant CHO cell line (CHR C5) and in its drug-sensitive parent (Aux B1) was studied in vitro using a clonogenic assay. The cytotoxicity of melphalan was significantly enhanced at elevated but non-lethal temperatures (39-41 degrees C) and hyperthermia potentiated the effect of melphalan in the lethal temperature range (43-44 degrees C) in both cell lines. The effect of temperature on membrane permeability to melphalan was studied to determine whether the increase in cytotoxicity was associated with increased intracellular drug levels. The uptake of 14C-labelled melphalan during 5 min increased with increasing temperature. Drug efflux, however, also increased at elevated temperatures. Intracellular drug levels at equilibrium were increased at elevated temperatures but the magnitude of this effect was small in comparison with the much larger increases in cytotoxicity. Nature Publishing Group 1990-08 /pmc/articles/PMC1971810/ /pubmed/1974814 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Bates, D. A. Mackillop, W. J. The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro. |
title | The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro. |
title_full | The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro. |
title_fullStr | The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro. |
title_full_unstemmed | The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro. |
title_short | The effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant CHO cells in vitro. |
title_sort | effect of hyperthermia in combination with melphalan on drug-sensitive and drug-resistant cho cells in vitro. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971810/ https://www.ncbi.nlm.nih.gov/pubmed/1974814 |
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