An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering

A novel method to link a nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was developed. The mRNA harboring amber stop codon at just downstream of initiation site was hybridized with hydrazide-modified ssDNA at upstream of coding region and was ligated to the DNA....

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Detalles Bibliográficos
Autores principales: Ueno, Shingo, Arai, Hidenao, Suzuki, Miho, Husimi, Yuzuru
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2007
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1975775/
https://www.ncbi.nlm.nih.gov/pubmed/17848981

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1975775/
https://www.ncbi.nlm.nih.gov/pubmed/17848981

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