Cargando…

Active site binding and sequence requirements for inhibition of HIV-1 reverse transcriptase by the RT1 family of single-stranded DNA aptamers

Nucleic acid aptamers can potentially be developed as broad-spectrum antiviral agents. Single-stranded DNA (ssDNA) aptamer RT1t49 inhibits reverse transcriptases (RT) from HIV-1 and diverse lentiviral subtypes with low nanomolar values of Kd and IC(50). To dissect the structural requirements for inh...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kissel, Jay D., Held, Daniel M., Hardy, Richard W., Burke, Donald H.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976467/ https://www.ncbi.nlm.nih.gov/pubmed/17644816 http://dx.doi.org/10.1093/nar/gkm420

Ejemplares similares

Novel bimodular DNA aptamers with guanosine quadruplexes inhibit phylogenetically diverse HIV-1 reverse transcriptases
por: Michalowski, Daniel, et al.
Publicado: (2008)

Binding interface and impact on protease cleavage for an RNA aptamer to HIV-1 reverse transcriptase
por: Nguyen, Phuong D M, et al.
Publicado: (2020)

HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors
por: Fisher, Timothy S, et al.
Publicado: (2005)

Potent Inhibition of HIV-1 Reverse Transcriptase and Replication by Nonpseudoknot, “UCAA-motif” RNA Aptamers
por: Whatley, Angela S, et al.
Publicado: (2013)

Aptamers that recognize drug-resistant HIV-1 reverse transcriptase
por: Li, Na, et al.
Publicado: (2008)

Aptamers that recognize drug-resistant HIV-1 reverse transcriptase
por: Li, Na, et al.
Publicado: (2009)

Specific binding of a hexanucleotide to HIV-1 reverse transcriptase: a novel class of bioactive molecules
por: Mescalchin, Alessandra, et al.
Publicado: (2006)

Broad-spectrum aptamer inhibitors of HIV reverse transcriptase closely mimic natural substrates
por: Ditzler, Mark A., et al.
Publicado: (2011)

Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity
por: Alves Ferreira-Bravo, Irani, et al.
Publicado: (2015)

Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer
por: Tuske, Steve, et al.
Publicado: (2020)

Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage
por: Penno, Christophe, et al.
Publicado: (2017)

RNA–protein interactions govern antiviral specificity and encapsidation of broad spectrum anti-HIV reverse transcriptase aptamers
por: Lange, Margaret J., et al.
Publicado: (2017)

Pausing kinetics dominates strand-displacement polymerization by reverse transcriptase
por: Malik, Omri, et al.
Publicado: (2017)

Rolling circle replication requires single-stranded DNA binding protein to avoid termination and production of double-stranded DNA
por: Ducani, Cosimo, et al.
Publicado: (2014)

NMR characterization of HIV-1 reverse transcriptase binding to various non-nucleoside reverse transcriptase inhibitors with different activities
por: Thammaporn, Ratsupa, et al.
Publicado: (2015)

Two Coselected Distal Mutations in HIV-1 Reverse Transcriptase (RT) Alter Susceptibility to Nonnucleoside RT Inhibitors and Nucleoside Analogs
por: Boyer, Paul L., et al.
Publicado: (2019)

Comparison of Reverse Transcriptase (RT) Activities of Various M-MuLV RTs for RT-LAMP Assays
por: Oscorbin, Igor P., et al.
Publicado: (2022)

The mutation T477A in HIV-1 reverse transcriptase (RT) restores normal proteolytic processing of RT in virus with Gag-Pol mutated in the p51-RNH cleavage site
por: Abram, Michael E, et al.
Publicado: (2010)

Site directed mutagenesis studies on HIV-1 reverse transcriptase (RT) shed light on the mechanism of action of a new Ribonuclease H/ DNA polymerase RT dual inhibitor
por: Corona, Angela, et al.
Publicado: (2013)

Mutational analysis of the HIV-1 auxiliary protein Vif identifies independent domains important for the physical and functional interaction with HIV-1 reverse transcriptase
por: Kataropoulou, Alexandra, et al.
Publicado: (2009)

Structural Studies and Structure Activity Relationships for Novel Computationally Designed Non-nucleoside Inhibitors and Their Interactions With HIV-1 Reverse Transcriptase
por: Frey, Kathleen M., et al.
Publicado: (2022)

Insights into the binding mode of AS1411 aptamer to nucleolin
por: Bie, Lihua, et al.
Publicado: (2022)

Effect of reverse transcriptase inhibitors on LINE-1 and Ty1 reverse transcriptase activities and on LINE-1 retrotransposition
por: Dai, Lixin, et al.
Publicado: (2011)

Differential expression of telomerase reverse transcriptase (hTERT) in lung tumours
por: Lantuejoul, S, et al.
Publicado: (2004)

Interactions between HIV-1 Reverse Transcriptase and the Downstream Template Strand in Stable Complexes with Primer-Template
por: Rutvisuttinunt, Wiriya, et al.
Publicado: (2008)

HIV-1 Uncoating and Reverse Transcription Require eEF1A Binding to Surface-Exposed Acidic Residues of the Reverse Transcriptase Thumb Domain
por: Rawle, Daniel J., et al.
Publicado: (2018)

A novel motif in telomerase reverse transcriptase regulates telomere repeat addition rate and processivity
por: Xie, Mingyi, et al.
Publicado: (2010)

The predictive and prognostic potential of plasma telomerase reverse transcriptase (TERT) RNA in rectal cancer patients
por: Rampazzo, Enrica, et al.
Publicado: (2018)

Insights into HIV-1 Reverse Transcriptase (RT) Inhibition and Drug Resistance from Thirty Years of Structural Studies
por: Singh, Abhimanyu K., et al.
Publicado: (2022)

In vitro selection and characterization of cellulose-binding DNA aptamers
por: Boese, Benjamin J., et al.
Publicado: (2007)

Specific implications of the HIV-1 nucleocapsid zinc fingers in the annealing of the primer binding site complementary sequences during the obligatory plus strand transfer
por: Godet, Julien, et al.
Publicado: (2011)

2′-fluoro-modified pyrimidines enhance affinity of RNA oligonucleotides to HIV-1 reverse transcriptase
por: Gruenke, Paige R., et al.
Publicado: (2020)

Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
por: Rutschke, Nils, et al.
Publicado: (2015)

Unexpected similarity between HIV-1 reverse transcriptase and tumor necrosis factor binding sites revealed by computer vision
por: Eguida, Merveille, et al.
Publicado: (2021)

AZT resistance alters enzymatic properties and creates an ATP-binding site in SFVmac reverse transcriptase
por: Schneider, Anna, et al.
Publicado: (2015)

Variation of Human Immunodeficiency Virus Type-1 Reverse Transcriptase within the Simian Immunodeficiency Virus Genome of RT-SHIV
por: Wadford, Debra A., et al.
Publicado: (2014)

Selection of RNA aptamers that bind HIV-1 LTR DNA duplexes: strand invaders
por: Srisawat, Chatchawan, et al.
Publicado: (2010)

Theoretical investigation of the interactions in binding pocket of Reverse Transcriptase
por: Sahu, Kamlesh Kumar, et al.
Publicado: (2015)

Specific reverse transcriptase slippage at the HIV ribosomal frameshift sequence: potential implications for modulation of GagPol synthesis
por: Penno, Christophe, et al.
Publicado: (2017)

The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells
por: Al-Minawi, Ali Z., et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_cvdkcam3hbhotle4u5h125tckt