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Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system

Solutions of tryptophan and N-hydroxypyridine-2-thione (mercaptopyridine-N-oxide, MPNO) were irradiated at 335 nm. Formation of 5-hydroxytryptophan was inferred from increased fluorescence at 334 nm on excitation at 315 nm, conditions chosen for selective detection of 5-hydroxytryptophan. Such exper...

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Detalles Bibliográficos
Autores principales: Botchway, Stanley W., Crisostomo, Ana G., Parker, Anthony W., Bisby, Roger H.
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1995038/
https://www.ncbi.nlm.nih.gov/pubmed/17553455
http://dx.doi.org/10.1016/j.abb.2007.04.026