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Sensitive Detection of p65 Homodimers Using Red-Shifted and Fluorescent Protein-Based FRET Couples

BACKGROUND: Fluorescence Resonance Energy Transfer (FRET) between the green fluorescent protein (GFP) variants CFP and YFP is widely used for the detection of protein-protein interactions. Nowadays, several monomeric red-shifted fluorescent proteins are available that potentially improve the efficie...

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Detalles Bibliográficos
Autores principales: Goedhart, Joachim, Vermeer, Joop E. M., Adjobo-Hermans, Merel J. W., van Weeren, Laura, Gadella, Theodorus W. J.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1995760/
https://www.ncbi.nlm.nih.gov/pubmed/17925859
http://dx.doi.org/10.1371/journal.pone.0001011