Microarray amplification bias: loss of 30% differentially expressed genes due to long probe – poly(A)-tail distances

BACKGROUND: Laser microdissection microscopy has become a rising tool to assess gene expression profiles of pure cell populations. Given the low yield of RNA, a second round of amplification is usually mandatory to yield sufficient amplified-RNA for microarray approaches. Since amplification induces...

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Detalles Bibliográficos
Autores principales: Boelens, Mirjam C, te Meerman, Gerard J, Gibcus, Johan H, Blokzijl, Tjasso, Boezen, H Marike, Timens, Wim, Postma, Dirkje S, Groen, Harry JM, van den Berg, Anke
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2000903/
https://www.ncbi.nlm.nih.gov/pubmed/17697374
http://dx.doi.org/10.1186/1471-2164-8-277

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2000903/
https://www.ncbi.nlm.nih.gov/pubmed/17697374
http://dx.doi.org/10.1186/1471-2164-8-277

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