Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).

The effects of cell density on drug-induced cell kill kinetics were studied by means of clonogenic assay using 3 human leukaemia-lymphoma cell lines. Mitoxantrone, daunorubicin, doxorubicin, vincristine and bleomycin were progressively less efficacious when cell density increased (positive inoculum...

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Autores principales: Ohnuma, T., Arkin, H., Holland, J. F.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1986
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001630/
https://www.ncbi.nlm.nih.gov/pubmed/2428392
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author Ohnuma, T.
Arkin, H.
Holland, J. F.
author_facet Ohnuma, T.
Arkin, H.
Holland, J. F.
author_sort Ohnuma, T.
collection PubMed
description The effects of cell density on drug-induced cell kill kinetics were studied by means of clonogenic assay using 3 human leukaemia-lymphoma cell lines. Mitoxantrone, daunorubicin, doxorubicin, vincristine and bleomycin were progressively less efficacious when cell density increased (positive inoculum effects), whereas the effects of cis-platin and carboplatin were not influenced by cell density. Inoculum effects were related to the kind of chemotherapeutic agents tested, irrespective of the type of cell lines used. Preincubation of mitoxantrone or doxorubicin in the presence of cells in high density resulted in decreases in the cytocidal activity, whereas the effects of bleomycin, vincristine and cis-platin were unaffected. These results show that cell density affects the biological effect of certain chemotherapeutic agents. Inactivation of drugs by high densities of cells partially explains this phenomenon.
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spelling pubmed-20016302009-09-10 Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect). Ohnuma, T. Arkin, H. Holland, J. F. Br J Cancer Research Article The effects of cell density on drug-induced cell kill kinetics were studied by means of clonogenic assay using 3 human leukaemia-lymphoma cell lines. Mitoxantrone, daunorubicin, doxorubicin, vincristine and bleomycin were progressively less efficacious when cell density increased (positive inoculum effects), whereas the effects of cis-platin and carboplatin were not influenced by cell density. Inoculum effects were related to the kind of chemotherapeutic agents tested, irrespective of the type of cell lines used. Preincubation of mitoxantrone or doxorubicin in the presence of cells in high density resulted in decreases in the cytocidal activity, whereas the effects of bleomycin, vincristine and cis-platin were unaffected. These results show that cell density affects the biological effect of certain chemotherapeutic agents. Inactivation of drugs by high densities of cells partially explains this phenomenon. Nature Publishing Group 1986-09 /pmc/articles/PMC2001630/ /pubmed/2428392 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Ohnuma, T.
Arkin, H.
Holland, J. F.
Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).
title Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).
title_full Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).
title_fullStr Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).
title_full_unstemmed Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).
title_short Effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).
title_sort effects of cell density on drug-induced cell kill kinetics in vitro (inoculum effect).
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2001630/
https://www.ncbi.nlm.nih.gov/pubmed/2428392
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AT hollandjf effectsofcelldensityondruginducedcellkillkineticsinvitroinoculumeffect

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