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Design of an improved set of oligonucleotide primers for genotyping MeCP2(tm1.1Bird )KO mice by PCR

BACKGROUND: The strain of MeCP2(tm1.1Bird )mice is a broadly used model for Rett syndrome. Because males carrying the invalidated MeCP2 locus are sterile, this strain has to be maintained in a heterozygous state. All animals therefore have to be genotyped at every generation to discriminate those ca...

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Detalles Bibliográficos
Autores principales: Miralvès, Julie, Magdeleine, Eddy, Joly, Etienne
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2018688/
https://www.ncbi.nlm.nih.gov/pubmed/17764542
http://dx.doi.org/10.1186/1750-1326-2-16