Development and application of a radioimmunoassay for methotrexate.

An antiserum to methotrexate has been produced in a sheep against a conjugate of ovalbumin and methotrexate (MTX) prepared using a water-soluble carbodiimide. The antibodies produced were specific for substances containing the 2,4-diamino pteridine structure. Naturally occurring folates did not inte...

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Detalles Bibliográficos
Autores principales: Aherne, G. W., Piall, E. M., Marks, V.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1977
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025394/
https://www.ncbi.nlm.nih.gov/pubmed/588422
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author Aherne, G. W.
Piall, E. M.
Marks, V.
author_facet Aherne, G. W.
Piall, E. M.
Marks, V.
author_sort Aherne, G. W.
collection PubMed
description An antiserum to methotrexate has been produced in a sheep against a conjugate of ovalbumin and methotrexate (MTX) prepared using a water-soluble carbodiimide. The antibodies produced were specific for substances containing the 2,4-diamino pteridine structure. Naturally occurring folates did not interfere with the assay. A radioimmunoassay has been developed using this antiserum, which can be used to measure MTX concentrations of less than 1 ng/ml in biological samples without prior extraction. The concentrations of MTX in the blood and urine of patients following a single i.v. bolus injection and following oral administration of the drug have been measured. The published radioimmunoassays for MTX have been compared.
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spelling pubmed-20253942009-09-10 Development and application of a radioimmunoassay for methotrexate. Aherne, G. W. Piall, E. M. Marks, V. Br J Cancer Research Article An antiserum to methotrexate has been produced in a sheep against a conjugate of ovalbumin and methotrexate (MTX) prepared using a water-soluble carbodiimide. The antibodies produced were specific for substances containing the 2,4-diamino pteridine structure. Naturally occurring folates did not interfere with the assay. A radioimmunoassay has been developed using this antiserum, which can be used to measure MTX concentrations of less than 1 ng/ml in biological samples without prior extraction. The concentrations of MTX in the blood and urine of patients following a single i.v. bolus injection and following oral administration of the drug have been measured. The published radioimmunoassays for MTX have been compared. Nature Publishing Group 1977-11 /pmc/articles/PMC2025394/ /pubmed/588422 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Aherne, G. W.
Piall, E. M.
Marks, V.
Development and application of a radioimmunoassay for methotrexate.
title Development and application of a radioimmunoassay for methotrexate.
title_full Development and application of a radioimmunoassay for methotrexate.
title_fullStr Development and application of a radioimmunoassay for methotrexate.
title_full_unstemmed Development and application of a radioimmunoassay for methotrexate.
title_short Development and application of a radioimmunoassay for methotrexate.
title_sort development and application of a radioimmunoassay for methotrexate.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025394/
https://www.ncbi.nlm.nih.gov/pubmed/588422
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