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Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the p...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025649/ https://www.ncbi.nlm.nih.gov/pubmed/17660318 http://dx.doi.org/10.1529/biophysj.107.112201 |