Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the p...

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Detalles Bibliográficos
Autores principales: Egner, Alexander, Geisler, Claudia, von Middendorff, Claas, Bock, Hannes, Wenzel, Dirk, Medda, Rebecca, Andresen, Martin, Stiel, Andre C., Jakobs, Stefan, Eggeling, Christian, Schönle, Andreas, Hell, Stefan W.
Formato: Texto
Lenguaje:English
Publicado: The Biophysical Society 2007
Materias:
Spectroscopy, Imaging, Other Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025649/
https://www.ncbi.nlm.nih.gov/pubmed/17660318
http://dx.doi.org/10.1529/biophysj.107.112201
Descripción
Sumario:We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.

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