Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the p...

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Detalles Bibliográficos
Autores principales: Egner, Alexander, Geisler, Claudia, von Middendorff, Claas, Bock, Hannes, Wenzel, Dirk, Medda, Rebecca, Andresen, Martin, Stiel, Andre C., Jakobs, Stefan, Eggeling, Christian, Schönle, Andreas, Hell, Stefan W.
Formato: Texto
Lenguaje:English
Publicado: The Biophysical Society 2007
Materias:
Spectroscopy, Imaging, Other Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025649/
https://www.ncbi.nlm.nih.gov/pubmed/17660318
http://dx.doi.org/10.1529/biophysj.107.112201
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author Egner, Alexander
Geisler, Claudia
von Middendorff, Claas
Bock, Hannes
Wenzel, Dirk
Medda, Rebecca
Andresen, Martin
Stiel, Andre C.
Jakobs, Stefan
Eggeling, Christian
Schönle, Andreas
Hell, Stefan W.
author_facet Egner, Alexander
Geisler, Claudia
von Middendorff, Claas
Bock, Hannes
Wenzel, Dirk
Medda, Rebecca
Andresen, Martin
Stiel, Andre C.
Jakobs, Stefan
Eggeling, Christian
Schönle, Andreas
Hell, Stefan W.
author_sort Egner, Alexander
collection PubMed
description We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.
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spelling pubmed-20256492008-07-23 Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters Egner, Alexander Geisler, Claudia von Middendorff, Claas Bock, Hannes Wenzel, Dirk Medda, Rebecca Andresen, Martin Stiel, Andre C. Jakobs, Stefan Eggeling, Christian Schönle, Andreas Hell, Stefan W. Biophys J Spectroscopy, Imaging, Other Techniques We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm. The Biophysical Society 2007-11-01 2007-07-27 /pmc/articles/PMC2025649/ /pubmed/17660318 http://dx.doi.org/10.1529/biophysj.107.112201 Text en Copyright © 2007, Biophysical Society This is an Open Access article distributed under the terms of the Creative Commons-Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/2.0/), which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Spectroscopy, Imaging, Other Techniques
Egner, Alexander
Geisler, Claudia
von Middendorff, Claas
Bock, Hannes
Wenzel, Dirk
Medda, Rebecca
Andresen, Martin
Stiel, Andre C.
Jakobs, Stefan
Eggeling, Christian
Schönle, Andreas
Hell, Stefan W.
Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
title Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
title_full Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
title_fullStr Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
title_full_unstemmed Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
title_short Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
title_sort fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
topic Spectroscopy, Imaging, Other Techniques
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025649/
https://www.ncbi.nlm.nih.gov/pubmed/17660318
http://dx.doi.org/10.1529/biophysj.107.112201
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