A new enzymatic route for production of long 5'-phosphorylated oligonucleotides using suicide cassettes and rolling circle DNA synthesis

BACKGROUND: The quality of chemically synthesized oligonucleotides falls with the length of the oligonucleotide, not least due to depurinations and premature termination during production. This limits the use of long oligonucleotides in assays where long high-quality oligonucleotides are needed (e.g...

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Detalles Bibliográficos
Autores principales: Lohmann, Jakob S, Stougaard, Magnus, Koch, Jørn
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040145/
https://www.ncbi.nlm.nih.gov/pubmed/17705815
http://dx.doi.org/10.1186/1472-6750-7-49

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040145/
https://www.ncbi.nlm.nih.gov/pubmed/17705815
http://dx.doi.org/10.1186/1472-6750-7-49

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