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Optical mapping as a routine tool for bacterial genome sequence finishing

BACKGROUND: In sequencing the genomes of two Xenorhabdus species, we encountered a large number of sequence repeats and assembly anomalies that stalled finishing efforts. This included a stretch of about 12 Kb that is over 99.9% identical between the plasmid and chromosome of X. nematophila. RESULTS...

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Detalles Bibliográficos
Autores principales: Latreille, Phil, Norton, Stacie, Goldman, Barry S, Henkhaus, John, Miller, Nancy, Barbazuk, Brad, Bode, Helge B, Darby, Creg, Du, Zijin, Forst, Steve, Gaudriault, Sophie, Goodner, Brad, Goodrich-Blair, Heidi, Slater, Steven
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2045679/
https://www.ncbi.nlm.nih.gov/pubmed/17868451
http://dx.doi.org/10.1186/1471-2164-8-321