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The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin

Heterochromatin normally has prescribed chromosomal positions and must not encroach on adjacent regions. We demonstrate that the fission yeast protein Epe1 stabilises silent chromatin, preventing the oscillation of heterochromatin domains. Epe1 loss leads to two contrasting phenotypes: alleviation o...

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Detalles Bibliográficos
Autores principales: Trewick, Sarah C, Minc, Elsa, Antonelli, Richard, Urano, Takeshi, Allshire, Robin C
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048757/
https://www.ncbi.nlm.nih.gov/pubmed/17948055
http://dx.doi.org/10.1038/sj.emboj.7601892
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author Trewick, Sarah C
Minc, Elsa
Antonelli, Richard
Urano, Takeshi
Allshire, Robin C
author_facet Trewick, Sarah C
Minc, Elsa
Antonelli, Richard
Urano, Takeshi
Allshire, Robin C
author_sort Trewick, Sarah C
collection PubMed
description Heterochromatin normally has prescribed chromosomal positions and must not encroach on adjacent regions. We demonstrate that the fission yeast protein Epe1 stabilises silent chromatin, preventing the oscillation of heterochromatin domains. Epe1 loss leads to two contrasting phenotypes: alleviation of silencing within heterochromatin and expansion of silent chromatin into neighbouring euchromatin. Thus, we propose that Epe1 regulates heterochromatin assembly and disassembly, thereby affecting heterochromatin integrity, centromere function and chromosome segregation fidelity. Epe1 regulates the extent of heterochromatin domains at the level of chromatin, not via the RNAi pathway. Analysis of an ectopically silenced site suggests that heterochromatin oscillation occurs in the absence of heterochromatin boundaries. Epe1 requires predicted iron- and 2-oxyglutarate (2-OG)-binding residues for in vivo function, indicating that it is probably a 2-OG/Fe(II)-dependent dioxygenase. We suggest that, rather than being a histone demethylase, Epe1 may be a protein hydroxylase that affects the stability of a heterochromatin protein, or protein–protein interaction, to regulate the extent of heterochromatin domains. Thus, Epe1 ensures that heterochromatin is restricted to the domains to which it is targeted by RNAi.
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spelling pubmed-20487572007-11-01 The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin Trewick, Sarah C Minc, Elsa Antonelli, Richard Urano, Takeshi Allshire, Robin C EMBO J Article Heterochromatin normally has prescribed chromosomal positions and must not encroach on adjacent regions. We demonstrate that the fission yeast protein Epe1 stabilises silent chromatin, preventing the oscillation of heterochromatin domains. Epe1 loss leads to two contrasting phenotypes: alleviation of silencing within heterochromatin and expansion of silent chromatin into neighbouring euchromatin. Thus, we propose that Epe1 regulates heterochromatin assembly and disassembly, thereby affecting heterochromatin integrity, centromere function and chromosome segregation fidelity. Epe1 regulates the extent of heterochromatin domains at the level of chromatin, not via the RNAi pathway. Analysis of an ectopically silenced site suggests that heterochromatin oscillation occurs in the absence of heterochromatin boundaries. Epe1 requires predicted iron- and 2-oxyglutarate (2-OG)-binding residues for in vivo function, indicating that it is probably a 2-OG/Fe(II)-dependent dioxygenase. We suggest that, rather than being a histone demethylase, Epe1 may be a protein hydroxylase that affects the stability of a heterochromatin protein, or protein–protein interaction, to regulate the extent of heterochromatin domains. Thus, Epe1 ensures that heterochromatin is restricted to the domains to which it is targeted by RNAi. Nature Publishing Group 2007-11-14 2007-10-18 /pmc/articles/PMC2048757/ /pubmed/17948055 http://dx.doi.org/10.1038/sj.emboj.7601892 Text en Copyright © 2007, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-nd/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
spellingShingle Article
Trewick, Sarah C
Minc, Elsa
Antonelli, Richard
Urano, Takeshi
Allshire, Robin C
The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin
title The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin
title_full The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin
title_fullStr The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin
title_full_unstemmed The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin
title_short The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin
title_sort jmjc domain protein epe1 prevents unregulated assembly and disassembly of heterochromatin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048757/
https://www.ncbi.nlm.nih.gov/pubmed/17948055
http://dx.doi.org/10.1038/sj.emboj.7601892
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