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Apical targeting of syntaxin 3 is essential for epithelial cell polarity
In polarized epithelial cells, syntaxin 3 localizes to the apical plasma membrane and is involved in membrane fusion of apical trafficking pathways. We show that syntaxin 3 contains a necessary and sufficient apical targeting signal centered around a conserved FMDE motif. Mutation of any of three cr...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063918/ https://www.ncbi.nlm.nih.gov/pubmed/16785322 http://dx.doi.org/10.1083/jcb.200603132 |
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author | Sharma, Nikunj Low, Seng Hui Misra, Saurav Pallavi, Bhattaram Weimbs, Thomas |
author_facet | Sharma, Nikunj Low, Seng Hui Misra, Saurav Pallavi, Bhattaram Weimbs, Thomas |
author_sort | Sharma, Nikunj |
collection | PubMed |
description | In polarized epithelial cells, syntaxin 3 localizes to the apical plasma membrane and is involved in membrane fusion of apical trafficking pathways. We show that syntaxin 3 contains a necessary and sufficient apical targeting signal centered around a conserved FMDE motif. Mutation of any of three critical residues within this motif leads to loss of specific apical targeting. Modeling based on the known structure of syntaxin 1 revealed that these residues are exposed on the surface of a three-helix bundle. Syntaxin 3 targeting does not require binding to Munc18b. Instead, syntaxin 3 recruits Munc18b to the plasma membrane. Expression of mislocalized mutant syntaxin 3 in Madin-Darby canine kidney cells leads to basolateral mistargeting of apical membrane proteins, disturbance of tight junction formation, and loss of ability to form an organized polarized epithelium. These results indicate that SNARE proteins contribute to the overall specificity of membrane trafficking in vivo, and that the polarity of syntaxin 3 is essential for epithelial cell polarization. |
format | Text |
id | pubmed-2063918 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20639182007-11-29 Apical targeting of syntaxin 3 is essential for epithelial cell polarity Sharma, Nikunj Low, Seng Hui Misra, Saurav Pallavi, Bhattaram Weimbs, Thomas J Cell Biol Research Articles In polarized epithelial cells, syntaxin 3 localizes to the apical plasma membrane and is involved in membrane fusion of apical trafficking pathways. We show that syntaxin 3 contains a necessary and sufficient apical targeting signal centered around a conserved FMDE motif. Mutation of any of three critical residues within this motif leads to loss of specific apical targeting. Modeling based on the known structure of syntaxin 1 revealed that these residues are exposed on the surface of a three-helix bundle. Syntaxin 3 targeting does not require binding to Munc18b. Instead, syntaxin 3 recruits Munc18b to the plasma membrane. Expression of mislocalized mutant syntaxin 3 in Madin-Darby canine kidney cells leads to basolateral mistargeting of apical membrane proteins, disturbance of tight junction formation, and loss of ability to form an organized polarized epithelium. These results indicate that SNARE proteins contribute to the overall specificity of membrane trafficking in vivo, and that the polarity of syntaxin 3 is essential for epithelial cell polarization. The Rockefeller University Press 2006-06-19 /pmc/articles/PMC2063918/ /pubmed/16785322 http://dx.doi.org/10.1083/jcb.200603132 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Sharma, Nikunj Low, Seng Hui Misra, Saurav Pallavi, Bhattaram Weimbs, Thomas Apical targeting of syntaxin 3 is essential for epithelial cell polarity |
title | Apical targeting of syntaxin 3 is essential for epithelial cell polarity |
title_full | Apical targeting of syntaxin 3 is essential for epithelial cell polarity |
title_fullStr | Apical targeting of syntaxin 3 is essential for epithelial cell polarity |
title_full_unstemmed | Apical targeting of syntaxin 3 is essential for epithelial cell polarity |
title_short | Apical targeting of syntaxin 3 is essential for epithelial cell polarity |
title_sort | apical targeting of syntaxin 3 is essential for epithelial cell polarity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063918/ https://www.ncbi.nlm.nih.gov/pubmed/16785322 http://dx.doi.org/10.1083/jcb.200603132 |
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