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Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells
In polarized epithelial cells, newly synthesized membrane proteins are delivered on specific pathways to either the apical or basolateral domains, depending on the sorting motifs present in these proteins. Because myosin VI has been shown to facilitate secretory traffic in nonpolarized cells, we inv...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064115/ https://www.ncbi.nlm.nih.gov/pubmed/17403927 http://dx.doi.org/10.1083/jcb.200608126 |
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author | Au, Josephine Sui-Yan Puri, Claudia Ihrke, Gudrun Kendrick-Jones, John Buss, Folma |
author_facet | Au, Josephine Sui-Yan Puri, Claudia Ihrke, Gudrun Kendrick-Jones, John Buss, Folma |
author_sort | Au, Josephine Sui-Yan |
collection | PubMed |
description | In polarized epithelial cells, newly synthesized membrane proteins are delivered on specific pathways to either the apical or basolateral domains, depending on the sorting motifs present in these proteins. Because myosin VI has been shown to facilitate secretory traffic in nonpolarized cells, we investigated its role in biosynthetic trafficking pathways in polarized MDCK cells. We observed that a specific splice isoform of myosin VI with no insert in the tail domain is required for the polarized transport of tyrosine motif containing basolateral membrane proteins. Sorting of other basolateral or apical cargo, however, does not involve myosin VI. Site-directed mutagenesis indicates that a functional complex consisting of myosin VI, optineurin, and probably the GTPase Rab8 plays a role in the basolateral delivery of membrane proteins, whose sorting is mediated by the clathrin adaptor protein complex (AP) AP-1B. Our results suggest that myosin VI is a crucial component in the AP-1B–dependent biosynthetic sorting pathway to the basolateral surface in polarized epithelial cells. |
format | Text |
id | pubmed-2064115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20641152007-11-29 Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells Au, Josephine Sui-Yan Puri, Claudia Ihrke, Gudrun Kendrick-Jones, John Buss, Folma J Cell Biol Research Articles In polarized epithelial cells, newly synthesized membrane proteins are delivered on specific pathways to either the apical or basolateral domains, depending on the sorting motifs present in these proteins. Because myosin VI has been shown to facilitate secretory traffic in nonpolarized cells, we investigated its role in biosynthetic trafficking pathways in polarized MDCK cells. We observed that a specific splice isoform of myosin VI with no insert in the tail domain is required for the polarized transport of tyrosine motif containing basolateral membrane proteins. Sorting of other basolateral or apical cargo, however, does not involve myosin VI. Site-directed mutagenesis indicates that a functional complex consisting of myosin VI, optineurin, and probably the GTPase Rab8 plays a role in the basolateral delivery of membrane proteins, whose sorting is mediated by the clathrin adaptor protein complex (AP) AP-1B. Our results suggest that myosin VI is a crucial component in the AP-1B–dependent biosynthetic sorting pathway to the basolateral surface in polarized epithelial cells. The Rockefeller University Press 2007-04-09 /pmc/articles/PMC2064115/ /pubmed/17403927 http://dx.doi.org/10.1083/jcb.200608126 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Au, Josephine Sui-Yan Puri, Claudia Ihrke, Gudrun Kendrick-Jones, John Buss, Folma Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells |
title | Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells |
title_full | Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells |
title_fullStr | Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells |
title_full_unstemmed | Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells |
title_short | Myosin VI is required for sorting of AP-1B–dependent cargo to the basolateral domain in polarized MDCK cells |
title_sort | myosin vi is required for sorting of ap-1b–dependent cargo to the basolateral domain in polarized mdck cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064115/ https://www.ncbi.nlm.nih.gov/pubmed/17403927 http://dx.doi.org/10.1083/jcb.200608126 |
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