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Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
Efficient delivery of cells to target tissues is a major problem in cell therapy. We report that enhancing delivery of mesoangioblasts leads to a complete reconstitution of downstream skeletal muscles in a mouse model of severe muscular dystrophy (α-sarcoglycan ko). Mesoangioblasts, vessel-associate...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064183/ https://www.ncbi.nlm.nih.gov/pubmed/16831885 http://dx.doi.org/10.1083/jcb.200512085 |
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author | Galvez, Beatriz G. Sampaolesi, Maurilio Brunelli, Silvia Covarello, Diego Gavina, Manuela Rossi, Barbara Costantin, Gabriela Torrente, Yvan Cossu, Giulio |
author_facet | Galvez, Beatriz G. Sampaolesi, Maurilio Brunelli, Silvia Covarello, Diego Gavina, Manuela Rossi, Barbara Costantin, Gabriela Torrente, Yvan Cossu, Giulio |
author_sort | Galvez, Beatriz G. |
collection | PubMed |
description | Efficient delivery of cells to target tissues is a major problem in cell therapy. We report that enhancing delivery of mesoangioblasts leads to a complete reconstitution of downstream skeletal muscles in a mouse model of severe muscular dystrophy (α-sarcoglycan ko). Mesoangioblasts, vessel-associated stem cells, were exposed to several cytokines, among which stromal- derived factor (SDF) 1 or tumor necrosis factor (TNF) α were the most potent in enhancing transmigration in vitro and migration into dystrophic muscle in vivo. Transient expression of α4 integrins or L-selectin also increased several fold migration both in vitro and in vivo. Therefore, combined pretreatment with SDF-1 or TNF-α and expression of α4 integrin leads to massive colonization (>50%) followed by reconstitution of >80% of α-sarcoglycan–expressing fibers, with a fivefold increase in efficiency in comparison with control cells. This study defines the requirements for efficient engraftment of mesoangioblasts and offers a new potent tool to optimize future cell therapy protocols for muscular dystrophies. |
format | Text |
id | pubmed-2064183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20641832007-11-29 Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability Galvez, Beatriz G. Sampaolesi, Maurilio Brunelli, Silvia Covarello, Diego Gavina, Manuela Rossi, Barbara Costantin, Gabriela Torrente, Yvan Cossu, Giulio J Cell Biol Research Articles Efficient delivery of cells to target tissues is a major problem in cell therapy. We report that enhancing delivery of mesoangioblasts leads to a complete reconstitution of downstream skeletal muscles in a mouse model of severe muscular dystrophy (α-sarcoglycan ko). Mesoangioblasts, vessel-associated stem cells, were exposed to several cytokines, among which stromal- derived factor (SDF) 1 or tumor necrosis factor (TNF) α were the most potent in enhancing transmigration in vitro and migration into dystrophic muscle in vivo. Transient expression of α4 integrins or L-selectin also increased several fold migration both in vitro and in vivo. Therefore, combined pretreatment with SDF-1 or TNF-α and expression of α4 integrin leads to massive colonization (>50%) followed by reconstitution of >80% of α-sarcoglycan–expressing fibers, with a fivefold increase in efficiency in comparison with control cells. This study defines the requirements for efficient engraftment of mesoangioblasts and offers a new potent tool to optimize future cell therapy protocols for muscular dystrophies. The Rockefeller University Press 2006-07-17 /pmc/articles/PMC2064183/ /pubmed/16831885 http://dx.doi.org/10.1083/jcb.200512085 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Galvez, Beatriz G. Sampaolesi, Maurilio Brunelli, Silvia Covarello, Diego Gavina, Manuela Rossi, Barbara Costantin, Gabriela Torrente, Yvan Cossu, Giulio Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability |
title | Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability |
title_full | Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability |
title_fullStr | Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability |
title_full_unstemmed | Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability |
title_short | Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability |
title_sort | complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064183/ https://www.ncbi.nlm.nih.gov/pubmed/16831885 http://dx.doi.org/10.1083/jcb.200512085 |
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