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Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability

Efficient delivery of cells to target tissues is a major problem in cell therapy. We report that enhancing delivery of mesoangioblasts leads to a complete reconstitution of downstream skeletal muscles in a mouse model of severe muscular dystrophy (α-sarcoglycan ko). Mesoangioblasts, vessel-associate...

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Autores principales: Galvez, Beatriz G., Sampaolesi, Maurilio, Brunelli, Silvia, Covarello, Diego, Gavina, Manuela, Rossi, Barbara, Costantin, Gabriela, Torrente, Yvan, Cossu, Giulio
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064183/
https://www.ncbi.nlm.nih.gov/pubmed/16831885
http://dx.doi.org/10.1083/jcb.200512085
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author Galvez, Beatriz G.
Sampaolesi, Maurilio
Brunelli, Silvia
Covarello, Diego
Gavina, Manuela
Rossi, Barbara
Costantin, Gabriela
Torrente, Yvan
Cossu, Giulio
author_facet Galvez, Beatriz G.
Sampaolesi, Maurilio
Brunelli, Silvia
Covarello, Diego
Gavina, Manuela
Rossi, Barbara
Costantin, Gabriela
Torrente, Yvan
Cossu, Giulio
author_sort Galvez, Beatriz G.
collection PubMed
description Efficient delivery of cells to target tissues is a major problem in cell therapy. We report that enhancing delivery of mesoangioblasts leads to a complete reconstitution of downstream skeletal muscles in a mouse model of severe muscular dystrophy (α-sarcoglycan ko). Mesoangioblasts, vessel-associated stem cells, were exposed to several cytokines, among which stromal- derived factor (SDF) 1 or tumor necrosis factor (TNF) α were the most potent in enhancing transmigration in vitro and migration into dystrophic muscle in vivo. Transient expression of α4 integrins or L-selectin also increased several fold migration both in vitro and in vivo. Therefore, combined pretreatment with SDF-1 or TNF-α and expression of α4 integrin leads to massive colonization (>50%) followed by reconstitution of >80% of α-sarcoglycan–expressing fibers, with a fivefold increase in efficiency in comparison with control cells. This study defines the requirements for efficient engraftment of mesoangioblasts and offers a new potent tool to optimize future cell therapy protocols for muscular dystrophies.
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spelling pubmed-20641832007-11-29 Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability Galvez, Beatriz G. Sampaolesi, Maurilio Brunelli, Silvia Covarello, Diego Gavina, Manuela Rossi, Barbara Costantin, Gabriela Torrente, Yvan Cossu, Giulio J Cell Biol Research Articles Efficient delivery of cells to target tissues is a major problem in cell therapy. We report that enhancing delivery of mesoangioblasts leads to a complete reconstitution of downstream skeletal muscles in a mouse model of severe muscular dystrophy (α-sarcoglycan ko). Mesoangioblasts, vessel-associated stem cells, were exposed to several cytokines, among which stromal- derived factor (SDF) 1 or tumor necrosis factor (TNF) α were the most potent in enhancing transmigration in vitro and migration into dystrophic muscle in vivo. Transient expression of α4 integrins or L-selectin also increased several fold migration both in vitro and in vivo. Therefore, combined pretreatment with SDF-1 or TNF-α and expression of α4 integrin leads to massive colonization (>50%) followed by reconstitution of >80% of α-sarcoglycan–expressing fibers, with a fivefold increase in efficiency in comparison with control cells. This study defines the requirements for efficient engraftment of mesoangioblasts and offers a new potent tool to optimize future cell therapy protocols for muscular dystrophies. The Rockefeller University Press 2006-07-17 /pmc/articles/PMC2064183/ /pubmed/16831885 http://dx.doi.org/10.1083/jcb.200512085 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Galvez, Beatriz G.
Sampaolesi, Maurilio
Brunelli, Silvia
Covarello, Diego
Gavina, Manuela
Rossi, Barbara
Costantin, Gabriela
Torrente, Yvan
Cossu, Giulio
Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
title Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
title_full Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
title_fullStr Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
title_full_unstemmed Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
title_short Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
title_sort complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064183/
https://www.ncbi.nlm.nih.gov/pubmed/16831885
http://dx.doi.org/10.1083/jcb.200512085
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