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Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms

Axon initial segments (AISs) and nodes of Ranvier are sites of action potential generation and propagation, respectively. Both domains are enriched in sodium channels complexed with adhesion molecules (neurofascin [NF] 186 and NrCAM) and cytoskeletal proteins (ankyrin G and βIV spectrin). We show th...

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Autores principales: Dzhashiashvili, Yulia, Zhang, Yanqing, Galinska, Jolanta, Lam, Isabel, Grumet, Martin, Salzer, James L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064285/
https://www.ncbi.nlm.nih.gov/pubmed/17548513
http://dx.doi.org/10.1083/jcb.200612012
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author Dzhashiashvili, Yulia
Zhang, Yanqing
Galinska, Jolanta
Lam, Isabel
Grumet, Martin
Salzer, James L.
author_facet Dzhashiashvili, Yulia
Zhang, Yanqing
Galinska, Jolanta
Lam, Isabel
Grumet, Martin
Salzer, James L.
author_sort Dzhashiashvili, Yulia
collection PubMed
description Axon initial segments (AISs) and nodes of Ranvier are sites of action potential generation and propagation, respectively. Both domains are enriched in sodium channels complexed with adhesion molecules (neurofascin [NF] 186 and NrCAM) and cytoskeletal proteins (ankyrin G and βIV spectrin). We show that the AIS and peripheral nervous system (PNS) nodes both require ankyrin G but assemble by distinct mechanisms. The AIS is intrinsically specified; it forms independent of NF186, which is targeted to this site via intracellular interactions that require ankyrin G. In contrast, NF186 is targeted to the node, and independently cleared from the internode, by interactions of its ectodomain with myelinating Schwann cells. NF186 is critical for and initiates PNS node assembly by recruiting ankyrin G, which is required for the localization of sodium channels and the entire nodal complex. Thus, initial segments assemble from the inside out driven by the intrinsic accumulation of ankyrin G, whereas PNS nodes assemble from the outside in, specified by Schwann cells, which direct the NF186-dependent recruitment of ankyrin G.
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spelling pubmed-20642852007-12-04 Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms Dzhashiashvili, Yulia Zhang, Yanqing Galinska, Jolanta Lam, Isabel Grumet, Martin Salzer, James L. J Cell Biol Research Articles Axon initial segments (AISs) and nodes of Ranvier are sites of action potential generation and propagation, respectively. Both domains are enriched in sodium channels complexed with adhesion molecules (neurofascin [NF] 186 and NrCAM) and cytoskeletal proteins (ankyrin G and βIV spectrin). We show that the AIS and peripheral nervous system (PNS) nodes both require ankyrin G but assemble by distinct mechanisms. The AIS is intrinsically specified; it forms independent of NF186, which is targeted to this site via intracellular interactions that require ankyrin G. In contrast, NF186 is targeted to the node, and independently cleared from the internode, by interactions of its ectodomain with myelinating Schwann cells. NF186 is critical for and initiates PNS node assembly by recruiting ankyrin G, which is required for the localization of sodium channels and the entire nodal complex. Thus, initial segments assemble from the inside out driven by the intrinsic accumulation of ankyrin G, whereas PNS nodes assemble from the outside in, specified by Schwann cells, which direct the NF186-dependent recruitment of ankyrin G. The Rockefeller University Press 2007-06-04 /pmc/articles/PMC2064285/ /pubmed/17548513 http://dx.doi.org/10.1083/jcb.200612012 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Dzhashiashvili, Yulia
Zhang, Yanqing
Galinska, Jolanta
Lam, Isabel
Grumet, Martin
Salzer, James L.
Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms
title Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms
title_full Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms
title_fullStr Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms
title_full_unstemmed Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms
title_short Nodes of Ranvier and axon initial segments are ankyrin G–dependent domains that assemble by distinct mechanisms
title_sort nodes of ranvier and axon initial segments are ankyrin g–dependent domains that assemble by distinct mechanisms
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064285/
https://www.ncbi.nlm.nih.gov/pubmed/17548513
http://dx.doi.org/10.1083/jcb.200612012
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