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An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown

During prophase, vertebrate cells disassemble their nuclear envelope (NE) in the process of NE breakdown (NEBD). We have established an in vitro assay that uses mitotic Xenopus laevis egg extracts and semipermeabilized somatic cells bearing a green fluorescent protein–tagged NE marker to study the m...

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Detalles Bibliográficos
Autores principales: Mühlhäusser, Petra, Kutay, Ulrike
Formato: Texto
Lenguaje:English
Publicado: Rockefeller University Press|1 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064467/
https://www.ncbi.nlm.nih.gov/pubmed/17698605
http://dx.doi.org/10.1083/jcb.200703002