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Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site

Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-mRNAs and prevents their flow to the cytoplasm by tethering them near the site of transcription. Here we provide evidence that mRNA release from the transcription site requires the heptad repeat structure of the...

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Autores principales: Custódio, Noélia, Vivo, Maria, Antoniou, Michael, Carmo-Fonseca, Maria
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064756/
https://www.ncbi.nlm.nih.gov/pubmed/17938247
http://dx.doi.org/10.1083/jcb.200612109
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author Custódio, Noélia
Vivo, Maria
Antoniou, Michael
Carmo-Fonseca, Maria
author_facet Custódio, Noélia
Vivo, Maria
Antoniou, Michael
Carmo-Fonseca, Maria
author_sort Custódio, Noélia
collection PubMed
description Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-mRNAs and prevents their flow to the cytoplasm by tethering them near the site of transcription. Here we provide evidence that mRNA release from the transcription site requires the heptad repeat structure of the C-terminal domain (CTD) of RNA polymerase II. The mammalian CTD, which is essential for normal co-transcriptional maturation of mRNA precursors, comprises 52 heptad repeats. We show that a truncated CTD containing 31 repeats (heptads 1–23, 36–38, and 48–52) is sufficient to support transcription, splicing, cleavage, and polyadenylation. Yet, the resulting mRNAs are mostly retained in the vicinity of the gene after transcriptional shutoff. The retained mRNAs maintain the ability to recruit components of the exon junction complex and the nuclear exosome subunit Rrp6p, suggesting that binding of these proteins is not sufficient for RNA release. We propose that the missing heptads in the truncated CTD mutant are required for binding of proteins implicated in a final co-transcriptional maturation of spliced and 3′ end cleaved and polyadenylated mRNAs into export-competent ribonucleoprotein particles.
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spelling pubmed-20647562008-04-22 Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site Custódio, Noélia Vivo, Maria Antoniou, Michael Carmo-Fonseca, Maria J Cell Biol Research Articles Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-mRNAs and prevents their flow to the cytoplasm by tethering them near the site of transcription. Here we provide evidence that mRNA release from the transcription site requires the heptad repeat structure of the C-terminal domain (CTD) of RNA polymerase II. The mammalian CTD, which is essential for normal co-transcriptional maturation of mRNA precursors, comprises 52 heptad repeats. We show that a truncated CTD containing 31 repeats (heptads 1–23, 36–38, and 48–52) is sufficient to support transcription, splicing, cleavage, and polyadenylation. Yet, the resulting mRNAs are mostly retained in the vicinity of the gene after transcriptional shutoff. The retained mRNAs maintain the ability to recruit components of the exon junction complex and the nuclear exosome subunit Rrp6p, suggesting that binding of these proteins is not sufficient for RNA release. We propose that the missing heptads in the truncated CTD mutant are required for binding of proteins implicated in a final co-transcriptional maturation of spliced and 3′ end cleaved and polyadenylated mRNAs into export-competent ribonucleoprotein particles. The Rockefeller University Press 2007-10-22 /pmc/articles/PMC2064756/ /pubmed/17938247 http://dx.doi.org/10.1083/jcb.200612109 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Custódio, Noélia
Vivo, Maria
Antoniou, Michael
Carmo-Fonseca, Maria
Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
title Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
title_full Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
title_fullStr Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
title_full_unstemmed Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
title_short Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
title_sort splicing- and cleavage-independent requirement of rna polymerase ii ctd for mrna release from the transcription site
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064756/
https://www.ncbi.nlm.nih.gov/pubmed/17938247
http://dx.doi.org/10.1083/jcb.200612109
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