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Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site
Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-mRNAs and prevents their flow to the cytoplasm by tethering them near the site of transcription. Here we provide evidence that mRNA release from the transcription site requires the heptad repeat structure of the...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064756/ https://www.ncbi.nlm.nih.gov/pubmed/17938247 http://dx.doi.org/10.1083/jcb.200612109 |
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author | Custódio, Noélia Vivo, Maria Antoniou, Michael Carmo-Fonseca, Maria |
author_facet | Custódio, Noélia Vivo, Maria Antoniou, Michael Carmo-Fonseca, Maria |
author_sort | Custódio, Noélia |
collection | PubMed |
description | Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-mRNAs and prevents their flow to the cytoplasm by tethering them near the site of transcription. Here we provide evidence that mRNA release from the transcription site requires the heptad repeat structure of the C-terminal domain (CTD) of RNA polymerase II. The mammalian CTD, which is essential for normal co-transcriptional maturation of mRNA precursors, comprises 52 heptad repeats. We show that a truncated CTD containing 31 repeats (heptads 1–23, 36–38, and 48–52) is sufficient to support transcription, splicing, cleavage, and polyadenylation. Yet, the resulting mRNAs are mostly retained in the vicinity of the gene after transcriptional shutoff. The retained mRNAs maintain the ability to recruit components of the exon junction complex and the nuclear exosome subunit Rrp6p, suggesting that binding of these proteins is not sufficient for RNA release. We propose that the missing heptads in the truncated CTD mutant are required for binding of proteins implicated in a final co-transcriptional maturation of spliced and 3′ end cleaved and polyadenylated mRNAs into export-competent ribonucleoprotein particles. |
format | Text |
id | pubmed-2064756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20647562008-04-22 Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site Custódio, Noélia Vivo, Maria Antoniou, Michael Carmo-Fonseca, Maria J Cell Biol Research Articles Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-mRNAs and prevents their flow to the cytoplasm by tethering them near the site of transcription. Here we provide evidence that mRNA release from the transcription site requires the heptad repeat structure of the C-terminal domain (CTD) of RNA polymerase II. The mammalian CTD, which is essential for normal co-transcriptional maturation of mRNA precursors, comprises 52 heptad repeats. We show that a truncated CTD containing 31 repeats (heptads 1–23, 36–38, and 48–52) is sufficient to support transcription, splicing, cleavage, and polyadenylation. Yet, the resulting mRNAs are mostly retained in the vicinity of the gene after transcriptional shutoff. The retained mRNAs maintain the ability to recruit components of the exon junction complex and the nuclear exosome subunit Rrp6p, suggesting that binding of these proteins is not sufficient for RNA release. We propose that the missing heptads in the truncated CTD mutant are required for binding of proteins implicated in a final co-transcriptional maturation of spliced and 3′ end cleaved and polyadenylated mRNAs into export-competent ribonucleoprotein particles. The Rockefeller University Press 2007-10-22 /pmc/articles/PMC2064756/ /pubmed/17938247 http://dx.doi.org/10.1083/jcb.200612109 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Custódio, Noélia Vivo, Maria Antoniou, Michael Carmo-Fonseca, Maria Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site |
title | Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site |
title_full | Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site |
title_fullStr | Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site |
title_full_unstemmed | Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site |
title_short | Splicing- and cleavage-independent requirement of RNA polymerase II CTD for mRNA release from the transcription site |
title_sort | splicing- and cleavage-independent requirement of rna polymerase ii ctd for mrna release from the transcription site |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064756/ https://www.ncbi.nlm.nih.gov/pubmed/17938247 http://dx.doi.org/10.1083/jcb.200612109 |
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