Cargando…

Single-Pair FRET Microscopy Reveals Mononucleosome Dynamics

We applied spFRET microscopy for direct observation of intranucleosomal DNA dynamics. Mononucleosomes, reconstituted with DNA containing a FRET pair at the dyad axis and exit of the nucleosome core particle, were immobilized through a 30 bp DNA tether on a polyethyleneglycol functionalized slide and...

Descripción completa

Detalles Bibliográficos
Autores principales: Koopmans, W. J. A., Brehm, A., Logie, C., Schmidt, T., van Noort, J.
Formato: Texto
Lenguaje:English
Publicado: Springer US 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064943/
https://www.ncbi.nlm.nih.gov/pubmed/17609864
http://dx.doi.org/10.1007/s10895-007-0218-9