No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.

The radiation response of Epstein-Barr virus (EBV)-immortalised lymphoblastoid cell lines derive from Li-Fraumeni syndrome (LFS) and LFS-like individuals was investigated. Cells from all LFS and LFS-like cases showed an accumulation of p53 protein following 137Cs gamma-irradiation, which was associa...

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Detalles Bibliográficos
Autores principales: Williams, K. J., Heighway, J., Birch, J. M., Norton, J. D., Scott, D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1996
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074690/
https://www.ncbi.nlm.nih.gov/pubmed/8795570
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author Williams, K. J.
Heighway, J.
Birch, J. M.
Norton, J. D.
Scott, D.
author_facet Williams, K. J.
Heighway, J.
Birch, J. M.
Norton, J. D.
Scott, D.
author_sort Williams, K. J.
collection PubMed
description The radiation response of Epstein-Barr virus (EBV)-immortalised lymphoblastoid cell lines derive from Li-Fraumeni syndrome (LFS) and LFS-like individuals was investigated. Cells from all LFS and LFS-like cases showed an accumulation of p53 protein following 137Cs gamma-irradiation, which was associated with cell cycle arrest at the G1/S border. This response was indistinguishable from that seen in cells derived from normal individuals, and occurred in cases with missense mutations in the TP53 gene at codons 175, 180, 220 and 248 and also in two LFS-like individuals with no TP53 mutation. Previous studies using lymphocytes and fibroblasts from LFS individuals have demonstrated abnormal radiation responses in these cells. This suggest cell type specificity in the contribution of a mutant p53 protein to phenotype. IMAGES:
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spelling pubmed-20746902009-09-10 No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines. Williams, K. J. Heighway, J. Birch, J. M. Norton, J. D. Scott, D. Br J Cancer Research Article The radiation response of Epstein-Barr virus (EBV)-immortalised lymphoblastoid cell lines derive from Li-Fraumeni syndrome (LFS) and LFS-like individuals was investigated. Cells from all LFS and LFS-like cases showed an accumulation of p53 protein following 137Cs gamma-irradiation, which was associated with cell cycle arrest at the G1/S border. This response was indistinguishable from that seen in cells derived from normal individuals, and occurred in cases with missense mutations in the TP53 gene at codons 175, 180, 220 and 248 and also in two LFS-like individuals with no TP53 mutation. Previous studies using lymphocytes and fibroblasts from LFS individuals have demonstrated abnormal radiation responses in these cells. This suggest cell type specificity in the contribution of a mutant p53 protein to phenotype. IMAGES: Nature Publishing Group 1996-09 /pmc/articles/PMC2074690/ /pubmed/8795570 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Williams, K. J.
Heighway, J.
Birch, J. M.
Norton, J. D.
Scott, D.
No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.
title No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.
title_full No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.
title_fullStr No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.
title_full_unstemmed No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.
title_short No defect in G1/S cell cycle arrest in irradiated Li-Fraumeni lymphoblastoid cell lines.
title_sort no defect in g1/s cell cycle arrest in irradiated li-fraumeni lymphoblastoid cell lines.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074690/
https://www.ncbi.nlm.nih.gov/pubmed/8795570
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