Fluorescence of 2-aminopurine reveals rapid conformational changes in the RB69 DNA polymerase-primer/template complexes upon binding and incorporation of matched deoxynucleoside triphosphates

We have used 2-aminopurine (2AP) as a fluorescent probe in the template strand of a 13/20mer primer/template (D) to detect deoxynucleoside triphosphates (N)-dependent conformational changes exhibited by RB69 DNA polymerase (ED) complexes. The rates and amplitudes of fluorescence quenching depend hyp...

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Detalles Bibliográficos
Autores principales: Zhang, H., Cao, W., Zakharova, E., Konigsberg, W., De La Cruz, E. M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2094073/
https://www.ncbi.nlm.nih.gov/pubmed/17766250
http://dx.doi.org/10.1093/nar/gkm587

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2094073/
https://www.ncbi.nlm.nih.gov/pubmed/17766250
http://dx.doi.org/10.1093/nar/gkm587

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