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A pyrosequencing-tailored nucleotide barcode design unveils opportunities for large-scale sample multiplexing

Multiplexed high-throughput pyrosequencing is currently limited in complexity (number of samples sequenced in parallel), and in capacity (number of sequences obtained per sample). Physical-space segregation of the sequencing platform into a fixed number of channels allows limited multiplexing, but o...

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Detalles Bibliográficos
Autores principales: Parameswaran, Poornima, Jalili, Roxana, Tao, Li, Shokralla, Shadi, Gharizadeh, Baback, Ronaghi, Mostafa, Fire, Andrew Z.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2095802/
https://www.ncbi.nlm.nih.gov/pubmed/17932070
http://dx.doi.org/10.1093/nar/gkm760