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Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers

BACKGROUND: The highly heterogenic characteristic of viruses is the major obstacle to efficient DNA amplification. Taking advantage of the large number of virus DNA sequences in public databases to select conserved sites for primer design is an optimal way to tackle the difficulties in virus genome...

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Detalles Bibliográficos
Autores principales: Zhang, Qingrun, Wu, Guanghua, Richards, Elliott, Jia, Shan'gang, Zeng, Changqing
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099425/
https://www.ncbi.nlm.nih.gov/pubmed/17892576
http://dx.doi.org/10.1186/1743-422X-4-92