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IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates

BACKGROUND: Coxiella burnetii contains the IS1111 transposase which is present 20 times in the Nine Mile phase I (9Mi/I) genome. A single PCR primer that binds to each IS element, and primers specific to a region ~500-bp upstream of each of the 20 IS1111 elements were designed. The amplified product...

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Detalles Bibliográficos
Autores principales: Denison, Amy M, Thompson, Herbert A, Massung, Robert F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2104537/
https://www.ncbi.nlm.nih.gov/pubmed/17949485
http://dx.doi.org/10.1186/1471-2180-7-91