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A HISTOCHEMICAL ENZYME KINETIC SYSTEM APPLIED TO THE TRYPSIN-LIKE AMIDASE AND ESTERASE ACTIVITY IN HUMAN MAST CELLS
A method for the determination of enzyme kinetic constants V(m), K(m), and K(i) in a histochemical system has been devised. As a substitute for the reciprocal of the reaction velocity, the times necessary to reach a fixed amount of end product (the initial visible color) in a tissue site at various...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1963
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106221/ https://www.ncbi.nlm.nih.gov/pubmed/13961705 |