METHODS FOR THE PURIFICATION OF THYMUS NUCLEI AND THEIR APPLICATION TO STUDIES OF NUCLEAR PROTEIN SYNTHESIS

Procedures are described for the purification of calf thymus nuclei using mild hypotonit shock to break intact cells, and layering techniques to remove cytoplasmic debris. Ficolc (a high polymer of sucrose) was dissolved in isotonic sucrose to give dense solutions suitable for gradient centrifugatio...

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Detalles Bibliográficos
Autores principales: Allfrey, V. G., Littau, V. C., Mirsky, A. E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106442/
https://www.ncbi.nlm.nih.gov/pubmed/14153483

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106442/
https://www.ncbi.nlm.nih.gov/pubmed/14153483

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