Cargando…

ISOLATION OF SKELETAL MUSCLE NUCLEI

A method employing aqueous media for isolation of nuclei from rat skeletal muscle is described. The technique involves (a) mincing and then homogenizing in a 0.32 M sucrose-salt solution with a Potter-Elvehjem type homogenizer using a Delrin (an acetal resin) pestle and a carefully controlled, relat...

Descripción completa

Detalles Bibliográficos
Autores principales: Edelman, Jean C., Edelman, P. Michael, Knigge, Karl M., Schwartz, Irving L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106724/
https://www.ncbi.nlm.nih.gov/pubmed/4287141