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ULTRATHIN FROZEN SECTIONS : I. Methods and Ultrastructural Preservation

A relatively simple method for obtaining ultrathin, frozen sections for electron microscopy has been developed. Tissues, cultured cells, and bacteria may be employed. They are fixed in 1.25–4% glutaraldehyde for 1–4 hr, are washed overnight in buffer at 3°C, and are embedded in 20% thiolated gelatin...

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Detalles Bibliográficos
Autores principales: Bernhard, W., Leduc, Elizabeth H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107175/
https://www.ncbi.nlm.nih.gov/pubmed/4167504