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ULTRATHIN FROZEN SECTIONS : I. Methods and Ultrastructural Preservation
A relatively simple method for obtaining ultrathin, frozen sections for electron microscopy has been developed. Tissues, cultured cells, and bacteria may be employed. They are fixed in 1.25–4% glutaraldehyde for 1–4 hr, are washed overnight in buffer at 3°C, and are embedded in 20% thiolated gelatin...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1967
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107175/ https://www.ncbi.nlm.nih.gov/pubmed/4167504 |