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ULTRATHIN FROZEN SECTIONS : II. Demonstration of Enzymic Activity

Endogenous enzyme activity can be readily and routinely demonstrated in ultrathin, frozen sections for electron microscopy. The procedure employed to obtain the best structural preservation as well as enzyme activity in thin sections involved fixation in glutaraldehyde, embedding in thiolated gelati...

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Detalles Bibliográficos
Autores principales: Leduc, Elizabeth H., Bernhard, W., Holt, S. J., Tranzer, J. P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107187/
https://www.ncbi.nlm.nih.gov/pubmed/4293006