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COMBINED CYTOCHEMICAL AND ELECTRON MICROSCOPIC DEMONSTRATION OF β-GLUCURONIDASE ACTIVITY IN RAT LIVER WITH THE USE OF A SIMULTANEOUS COUPLING AZO DYE TECHNIQUE
Rat liver was fixed in formal-cacodylate-sucrose and frozen sections were incubated in a simultaneous-coupling medium containing naphthol AS-BI glucuronide as substrate and hexazonium pararosanilin as the diazo reagent. By light microscopy, the sections demonstrated β-glucuronidase activity as red d...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1968
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107359/ https://www.ncbi.nlm.nih.gov/pubmed/4170545 |