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HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS : A Biochemical and Fine Structural Study
A new technique employing continuous recirculating perfusion of the rat liver in situ, shaking of the liver in buffer in vitro, and filtration of the tissue through nylon mesh, results in the conversion of about 50% of the liver into intact, isolated parenchymal cells. The perfusion media consist of...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1969
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107801/ https://www.ncbi.nlm.nih.gov/pubmed/4900611 |