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CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONS : II. Location of the Polypeptides in Rough Microsomes
Rough microsomes were incubated in an in vitro amino acid-incorporating system for labeling the nascent polypeptide chains on the membrane-bound ribosomes. Sucrose density gradient analysis showed that ribosomes did not detach from the membranes during incorporation in vitro. Trypsin and chymotrypsi...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1970
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107985/ https://www.ncbi.nlm.nih.gov/pubmed/5458993 |