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CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONS : I. Location of the Polypeptides within Ribosomes

Free ribosomes containing nascent polypeptide chains labeled in vitro were submitted to proteolysis at 0° by a mixture of trypsin and chymotrypsin. Sucrose gradient analysis showed that polysome patterns are retained even after 24 hr of proteolysis in the cold, while messenger RNA-free ribosomes (ge...

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Detalles Bibliográficos
Autores principales: Blobel, G., Sabatini, D. D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107986/
https://www.ncbi.nlm.nih.gov/pubmed/5458992