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A METHOD FOR DETERMINING TOTAL PROTEIN OF ISOLATED CELLULAR ELEMENTS AND CORRESPONDING TRITIUM RADIOACTIVITY
A method is described for the microanalysis of protein, obtained from isolated tissue elements, in the range of 500 µµg-500 mµg. The method entails solubilization of cellular protein with phosphoric acid and heat after extraction of acid-soluble compounds, lipids, and RNA. A procedure for the extrac...
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| Formato: | Texto |
| Lenguaje: | English |
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The Rockefeller University Press
1968
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108371/ https://www.ncbi.nlm.nih.gov/pubmed/5664225 |